FastQCFastQC Report
Thu 26 May 2016
SRR2094106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8050986
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC452920.5625646349403662RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC238240.29591406568089923RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206370.2563288521430791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171030.21243360751093096No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA154890.19238637354480556RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG138910.172537873000897RNA PCR Primer, Index 27 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128880.16007977159567785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120100.029.8966331
GTATCAA221650.021.5686241
CGACCGT1251.772969E-819.79841219
ATCTCCG130900.017.444810
TGCTTGA43950.017.35700645
TATCAAC303250.015.6687872
CTGCTTG50750.015.65122644
ATCAACG302400.015.6309933
TCAACGC304350.015.5604124
TATCTCG146900.015.36335936
ACATCTC152500.015.3575948
GACGGAC148200.015.34843626
TGCCGTC143600.015.34130445
CTCGTAT146750.015.31649539
CAACGCA309700.015.2916095
TCTCGTA147350.015.27043738
TATGCCG147000.015.26087643
TCTCCGA150050.015.24840911
ACGGACT149150.015.22050727
CACGAGA150050.015.18853121
Produced by FastQC (version 0.11.5)