FastQCFastQC Report
Thu 26 May 2016
SRR2094105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607039
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC301130.34986480251803204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177940.20673776428804377No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC162080.18831098592675136RNA PCR Primer, Index 26 (100% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142020.16500448063497794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107630.12504881179230162No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA97460.11323290158206556RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG96050.11159470754111837RNA PCR Primer, Index 26 (100% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105700.031.467581
GTATCAA213000.021.4344231
ATCTCCG91600.017.51213510
TATGCCG98250.016.21450843
CTCGTAT98750.016.19973239
TCTCGTA98800.016.19228638
TCGTATG100350.015.94153140
CACGAGA101200.015.82876521
CGAGACT101100.015.82235123
CCCACGA101550.015.7966419
ATCTCGT102050.015.6989337
TGCCGTC100100.015.69078245
CCACGAG102850.015.68447420
TCCGAGC103400.015.64419913
CGTATGC102750.015.61287841
CGAGCCC103350.015.60859415
GCCCACG103250.015.6019218
ATGCCGT101600.015.54726644
ATCAACG289450.015.4987473
TATCAAC292300.015.4938722
Produced by FastQC (version 0.11.5)