FastQCFastQC Report
Thu 26 May 2016
SRR2094104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4598892
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62610.13614148799319487No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC60650.13187959186691053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49410.10743892224474938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.029.9583511
GTATCAA71450.021.0311991
ATCTCCG21750.015.61934310
ATCAACG97650.015.1369433
TATCAAC99550.015.07403852
TCAACGC99150.014.9306334
CAACGCA100950.014.731275
AACGCAG104500.014.273896
ACGCCCG2254.9149094E-913.99894521
AATCTCG23350.013.87717136
CTCGTAT23350.013.77960439
TCTCGTA23550.013.75901938
ACGAGAC24700.013.57185222
CCCACGA24600.013.53585919
TCCGAGC25350.013.4012113
TCTCCGA25500.013.32238111
CCACGAG25200.013.30285820
GCCCACG25100.013.26622118
TATGCCG24450.013.15994843
TGCCGTC24650.013.05402645
Produced by FastQC (version 0.11.5)