FastQCFastQC Report
Thu 26 May 2016
SRR2094103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6733061
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112360.1668780366017774No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC107810.16012033754038468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88710.13175285356838443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68890.1023160194152407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67350.029.4726561
GTATCAA135600.022.5476441
TCAACGC180400.016.549424
ATCAACG180900.016.5036773
TATCAAC184350.016.3778822
CAACGCA184450.016.198245
AACGCAG188950.015.8600936
TCGATCG1908.010829E-915.395741
ATCTCCG35700.014.30559910
ACGCAGA214600.013.9015187
CGCAGAG216350.013.7058798
TCTCGTA37050.013.42099538
AATCTCG37600.013.34376336
TATGCCG37800.013.27513343
TGCCGTC38250.013.17788245
CTCGTAT37850.013.13732839
CCCGTAT3950.013.10159926
GACGTAG38650.013.04043526
ACGTAGA39200.013.02966827
GAGTACT145250.012.88711112
Produced by FastQC (version 0.11.5)