FastQCFastQC Report
Thu 26 May 2016
SRR2094102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8768554
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405800.46279010199401177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337580.38498936084558527No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC277550.31652881421497775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269210.30701755386349905No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC152360.17375726944260136RNA PCR Primer, Index 33 (95% over 23bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA91250.10406504880964411RNA PCR Primer, Index 33 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193600.033.488061
CGACCGT2500.024.29785319
TCGACCG2450.022.95715518
GTATCAA392000.022.103741
GTTCGAC2850.020.52450216
ATCTCCG81450.016.71123110
CGCTCCG3750.016.19949234
TCTCGCG850.002567421915.88194736
TATCAAC550050.015.6940052
TATGCCG83550.015.61996943
TCTCGTA84600.015.58461938
CTCGTAT84350.015.52411439
ATCAACG555350.015.4470023
AATCTCG86550.015.441545536
TGCCGTC85250.015.33523145
TCAACGC561900.015.2309024
CACGAGA88650.015.22873121
ATCTCGT87200.015.17171837
CCCACGA89600.015.09074219
CGAGCCC90100.015.08190915
Produced by FastQC (version 0.11.5)