FastQCFastQC Report
Thu 26 May 2016
SRR2094101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8144072
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC269720.3311856771403789RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTC138930.17059033859229142RNA PCR Primer, Index 23 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93390.11467236537201539No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA92500.11357954595686286RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG81590.10018329896886963RNA PCR Primer, Index 23 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71850.027.6366921
GTATCAA141200.021.5654031
ATCTCCG76950.018.24378410
ATCAACG177250.016.7289283
CCGTACG952.8241577E-416.5772849
TCAACGC179750.016.4712264
TATCAAC182150.016.4641742
CAACGCA184100.016.0942575
TCTCCGA89200.015.83921911
CGAGACC88200.015.8167823
AACGCAG187950.015.7885216
CTCGTAT86100.015.73186839
TCTCGTA86700.015.674938
TGCCGTC86200.015.55843745
TATGCCG86900.015.5098443
CCCACGA90550.015.50369119
ACGAGAC90350.015.48982522
GCCCACG90550.015.42915318
GATCTCG89150.015.34442136
TCCGAGC91550.015.33434413
Produced by FastQC (version 0.11.5)