FastQCFastQC Report
Thu 26 May 2016
SRR2094100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6838022
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC222630.3255766068023765RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC133720.19555362647268462RNA PCR Primer, Index 39 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129240.18900202426959142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105020.1535824248591186No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA101370.14824462395704488RNA PCR Primer, Index 39 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83000.12138013010195053No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG77280.11301513800335827RNA PCR Primer, Index 39 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85800.031.014961
GTATCAA169600.021.3500391
ATCTCCG70100.016.17558910
TATCAAC236250.015.2749612
ATCAACG235950.015.0369353
TGCTTGA34100.014.9796545
TCAACGC238250.014.8917714
CAACGCA243000.014.5914185
AACGCAG246200.014.4200416
CGCAATT1605.03758E-614.06178134
TCTCGTA80750.013.820230538
TATGCCG81050.013.79754443
TATACAC97850.013.7725263
TCTCCGA82450.013.72539711
CGAGACC83150.013.69212223
CTCGTAT81500.013.63783739
TCGTATG82150.013.55771440
TGCCGTC81750.013.5427745
CGCGATA1000.00755055913.50148144
GTACCGT3009.094947E-1213.4989156
Produced by FastQC (version 0.11.5)