FastQCFastQC Report
Thu 26 May 2016
SRR2094099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5858578
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC162560.27747347564545527No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC94240.16085814680627278TruSeq Adapter, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75060.128119827029699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59210.1010654803947306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.029.7608911
GTATCAA106750.021.8295331
ATCTCCG50850.016.19319310
ATCAACG145050.015.6965863
TCAACGC146300.015.5470944
TATCAAC147550.015.4611262
CGAGACT53650.015.22369523
TCTCGTA53400.015.21095838
CAACGCA149600.015.1891065
CTCGTAT53850.015.12562939
TGCCGTC54250.015.01525845
TATGCCG54050.014.94566743
ATGCCGT54850.014.851006544
CCCACGA55150.014.84903619
GATCTCG56150.014.786056536
AACGCAG154350.014.76546
GCCCACG55900.014.69005718
TCCGAGC56750.014.66824813
ATCTCGT56400.014.64160437
CCACGAG55950.014.63696720
Produced by FastQC (version 0.11.5)