FastQCFastQC Report
Thu 26 May 2016
SRR2094098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335639
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC149680.23625083436729905RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108100.1706220951035878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89500.1412643618110186No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC73240.1156000207713855RNA PCR Primer, Index 27 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65230.10295725498248876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64100.030.4838181
GTATCAA131650.022.4263571
ATCTCCG44100.017.54915810
ATCAACG173600.016.5752743
TATCAAC174800.016.5258372
TCAACGC174950.016.447374
CAACGCA179050.016.0581825
CGAGCGT850.00256666815.88254831
AACGCAG181900.015.8065856
TATCTCG48100.015.71806836
TATGCCG48050.015.59393843
ACGGACT47950.015.57818127
TCTCGTA48550.015.47968838
GACGGAC49400.015.394196526
CGGACTC48750.015.36844928
CTCGTAT49250.015.30451539
TCGTATG49900.015.10515840
CGAGACG50050.015.01398523
TCTCCGA51800.014.85363611
TCCGAGC52000.014.83977113
Produced by FastQC (version 0.11.5)