FastQCFastQC Report
Thu 26 May 2016
SRR2094097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401314
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC59581.4846230134009777RNA PCR Primer, Index 26 (100% over 23bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA40901.0191520853994627RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG38640.9628370801915707RNA PCR Primer, Index 26 (100% over 25bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT18900.47095291965892044RNA PCR Primer, Index 26 (100% over 28bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA11260.2805783002835685RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC10750.2678700468959468RNA PCR Primer, Index 26 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC10730.2673716840180008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.171187648574433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.16794828986778432No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT5860.14602032323816264RNA PCR Primer, Index 26 (100% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT5090.12683335243724364RNA PCR Primer, Index 26 (95% over 23bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT4490.11188246609886524RNA PCR Primer, Index 26 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.10365947861275708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCC300.0051438729.99933411
TGCTTGA8350.029.64006245
TTAGTAC554.15678E-628.635733
CTGCTTG9300.026.85424444
GGTATCA4200.025.7393721
ACAGCCG450.001225321924.9994475
CTTATAC4200.024.6668991
CGTCCGA751.6933864E-623.99946811
GCTTGAA3100.023.95108245
GTATCAA8200.022.2472921
AGACCGC955.514412E-721.31531710
CGACATG855.019714E-621.17615
ATCCCGC855.019714E-621.17639
GTCCGAC855.019714E-621.17612
GAATGCG654.2322424E-420.76877211
GGATAGT654.2322424E-420.7687726
AATGCGT654.2322424E-420.76877212
CGTAGAG654.2322424E-420.76877216
TCTGCTT12150.020.74028243
GGACTGT1009.026717E-720.2495526
Produced by FastQC (version 0.11.5)