FastQCFastQC Report
Thu 26 May 2016
SRR2094096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6449599
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC155300.24079016385359772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86400.1339618168509391No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC78830.12222465303656863RNA PCR Primer, Index 17 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68050.10551043561002785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.028.3583451
GTATCAA117250.022.0092161
ATCTCCG46400.018.76404610
TATGCCG51400.016.67897843
ATCAACG151400.016.5686023
TCTCGTA52150.016.48225438
CTCGTAT52150.016.48110439
ATGCCGT52000.016.44312944
TCGTATG52300.016.43396440
TCCGAGC53150.016.42335913
TCAACGC152850.016.4114254
CGAGCCC53500.016.35847515
CCCACGA53250.016.35077519
TGCCGTC52350.016.29147545
ACGAGAC53600.016.24400522
CACGAGA53350.016.23540721
TATCAAC157850.016.2193932
TCTCCGA54000.016.1231811
AATCTCG53550.016.09398836
CAACGCA156600.016.0471655
Produced by FastQC (version 0.11.5)