FastQCFastQC Report
Thu 26 May 2016
SRR2094095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5492001
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC170930.3112344662719471No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC115740.21074286038913684TruSeq Adapter, Index 11 (95% over 22bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA84550.15395117371610093TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG67280.12250544018473411RNA PCR Primer, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66110.12037506912325763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.028.8312511
GTATCAA98700.022.0493771
AACGTCG807.5733646E-519.68624122
TATCAAC134000.016.0847932
ATCAACG133350.016.0450943
TCAACGC134000.015.9168924
ACGTCGT1004.1726461E-415.74899223
CAACGCA137500.015.5117355
ATCTCCG57600.015.38964110
AACGCAG140100.015.2399246
TGCTTGA28050.013.55787545
TCTCCGA67150.013.167438511
ACGCAGA160350.013.1609927
TATACAC79750.013.1464943
TCTCGTA66300.012.99820838
ACATCTC71750.012.9817498
CGTACTA66900.012.98135129
GACCGTA67000.012.96197526
CTCGTAT67000.012.92898439
ACCGTAC66850.012.92374927
Produced by FastQC (version 0.11.5)