FastQCFastQC Report
Thu 26 May 2016
SRR2094094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6526744
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC158880.24342918919448964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123950.18991092648953292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100260.15361411448035958No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC85740.1313671870690807RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73080.11197007267329621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78400.029.8163991
GTATCAA158900.022.8257261
GGCGAAT55700.016.60299732
ATCTCCG56800.016.55660210
ATCAACG218400.016.4305213
TATCAAC219950.016.4170192
GCGAATC56850.016.22755833
TCAACGC222100.016.1466714
CAACGCA224950.015.9621055
TAAGGCG57900.015.89307729
CGAATCT58250.015.76064734
AGGCGAA58850.015.6760731
CGAGACT58800.015.64945523
AACGCAG230250.015.6044516
CTCGTAT56450.015.58425439
TCTCGTA57000.015.55289538
TATGCCG56000.015.46853643
TCGTATG57200.015.37991540
AATCTCG59500.015.35390736
AAGGCGA60250.015.34810230
Produced by FastQC (version 0.11.5)