FastQCFastQC Report
Thu 26 May 2016
SRR2094093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8398939
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC239700.2853931907351631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162850.19389353821952987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129380.15404326665546683No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC124280.1479710711079102TruSeq Adapter, Index 3 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91540.10898995694575231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102200.031.379061
GTATCAA209450.021.93931
ATCTCCG69850.017.19969410
ATCAACG277450.016.290753
TATCAAC280550.016.2550892
TCAACGC277900.016.2238924
TATGCCG74550.016.05708543
CAACGCA283350.015.9277195
AACGCAG285900.015.7935256
TCTCGTA76050.015.76874538
CTCGTAT76350.015.76562839
AATCTCG76300.015.65772936
ATGCCGT76900.015.47916744
TCGTATG78050.015.45152540
CGTATGC78250.015.41249241
TGCCGTC77800.015.35785645
CCACGAG79150.015.34948720
CACGAGA78900.015.31348921
ACGTAGA77550.015.26183327
GACGTAG77800.015.18386826
Produced by FastQC (version 0.11.5)