FastQCFastQC Report
Thu 26 May 2016
SRR2094092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4810013
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80200.16673551609943674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69990.14550896224189No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC59540.123783449233921No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.10103922796050656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.030.8925111
GTATCAA96000.021.221721
ATCTCCG19600.015.95515810
TCTCGTA19000.015.86761438
CTCGTAT19150.015.86081339
TATCAAC126650.015.8098292
ATCAACG125000.015.7125483
TCAACGC125500.015.6320234
AATCTCG19500.015.576941536
TATGCCG19350.015.46545543
TGCCGTC19950.015.45291245
CAACGCA128400.015.2789615
ATGCCGT19900.015.2657444
ACGCCCG1806.886694E-815.00001621
AACGCAG130800.014.9642136
CGAGCCC21200.014.75099615
ACGAGAC20750.014.747004522
TCGTATG20450.014.63250840
CCCACGA21600.014.4779819
TCCGAGC22300.014.42691913
Produced by FastQC (version 0.11.5)