FastQCFastQC Report
Thu 26 May 2016
SRR2094091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7945544
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC216510.272492355463641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164870.2074999521744515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135180.17013309598436557No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTC98980.12457296819449998RNA PCR Primer, Index 31 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98660.12417022673337408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103200.031.5976451
GTATCAA209100.022.4815441
CGCTACG54600.018.83168628
CGCTATC53900.018.82579833
ACGCTAC55650.018.47811527
CTCGTAT55100.018.41637839
TATGCCG54950.018.34485243
GACGCTA56350.018.24857326
TGCCGTC55000.018.12441845
CCCACGA57450.018.05310619
TATCTCG56350.017.9674736
TACGCTA57200.017.93625331
ACGCTAT57150.017.83395232
TCGTATG57050.017.74779340
TCTCGTA56700.017.73807538
GCTACGC58650.017.68473429
ATGCCGT56450.017.65897844
CTACGCT58700.017.51635630
CACGAGA59700.017.48763721
GCCCACG59350.017.43725618
Produced by FastQC (version 0.11.5)