FastQCFastQC Report
Thu 26 May 2016
SRR2094090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7849851
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC182960.23307448765588035RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTC93660.11931436660390116RNA PCR Primer, Index 23 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82520.10512301443683454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65450.027.583321
GTATCAA125650.021.148281
ATCTCCG57950.017.1209610
ATCAACG162350.016.0610563
TCAACGC163050.015.9645064
TATCAAC166400.015.764792
CAACGCA167600.015.5579495
AACGCAG169950.015.3428196
CTCGTAT63550.015.18850839
TCTCGTA62900.015.16661138
TGCCGTC63650.014.98933245
TCTCCGA66500.014.95352411
TATGCCG64750.014.87378943
CGAGACC65650.014.73740223
ATGCCGT65550.014.62351544
ATCTCGT65800.014.56665537
ACGAGAC66950.014.55178322
TCGTATG66950.014.51808440
CCACGAG66950.014.48355420
CCGAGCC69600.014.4815314
Produced by FastQC (version 0.11.5)