FastQCFastQC Report
Thu 26 May 2016
SRR2094089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6268544
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC188440.3006120719580177No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC101840.16246196883997305TruSeq Adapter, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80150.12786063238927572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64490.10287875461989258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.029.5910471
GTATCAA113800.021.16981
ATCTCCG59200.016.72157710
ATCAACG149550.015.7358983
TATCAAC151200.015.6385752
TCAACGC150700.015.6158154
TCTCGTA63150.015.28473538
CGAGACT64500.015.24376523
CAACGCA154750.015.221675
CTCGTAT63700.015.046839
TATGCCG64050.015.0012643
ACGAGAC66200.014.95426922
TGCCGTC63850.014.94276245
ATGCCGT64300.014.87330144
AACGCAG159000.014.84316
CCCACGA66850.014.774384519
CCACGAG67250.014.75365120
GATCTCG65700.014.72538636
TCTCCGA67700.014.72181411
TCGTATG64950.014.68816840
Produced by FastQC (version 0.11.5)