FastQCFastQC Report
Thu 26 May 2016
SRR2094088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8328340
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC223100.26788051400399115RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147830.17750235941376072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121060.145359099172224No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC111900.13436050881688308RNA PCR Primer, Index 27 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84790.10180900395516992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92100.031.6689151
GTATCAA186150.022.9700971
ATCTCCG67050.016.84405310
TATCAAC258950.016.2902222
ATCAACG259600.016.2061023
TCAACGC261950.016.0607154
TATCTCG69250.016.0186636
TCGTATG69050.015.86953840
TATGCCG68950.015.85963543
ACGGACT69600.015.80746227
CGGACTC69650.015.76352728
CAACGCA267100.015.7257775
CTCGTAT69750.015.67773239
GACGGAC70250.015.66120126
TCTCGTA69900.015.64446538
CGAGACG71950.015.57231823
CGTATGC71250.015.50529341
TGCCGTC71050.015.45458545
CACGAGA73100.015.32715121
ACGAGAC73350.015.30567622
Produced by FastQC (version 0.11.5)