FastQCFastQC Report
Thu 26 May 2016
SRR2094087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6280763
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC134410.21400266177851324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83490.13292970933627013No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC71890.11446061569271122RNA PCR Primer, Index 17 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66380.10568779621202073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.027.3302881
GTATCAA110300.022.5197941
ATCAACG141450.017.1935023
TCAACGC141900.017.0914134
TATCAAC144300.017.0566082
CAACGCA144750.016.7082735
AACGCAG148000.016.386976
ATCTCCG42650.016.08875510
TCTCGTA47150.014.36444138
TCTCCGA48300.014.25331711
CTCGTAT48000.014.109282539
TCGTATG47850.014.05969340
TCCGAGC49450.014.05833313
TATGCCG47750.014.04235343
ACGCAGA171150.014.0258487
CGAGCCC49150.013.96115715
CGCAGAG173200.013.8338598
ATGCCGT48350.013.82166544
AATCTCG48850.013.8188236
CACGAGA49350.013.72222221
Produced by FastQC (version 0.11.5)