FastQCFastQC Report
Thu 26 May 2016
SRR2094086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4861582
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC107760.2216562427621297No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC73860.15192585458807442TruSeq Adapter, Index 11 (95% over 22bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA56930.11710179937312587TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52700.1084009279283986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.028.6864781
GTATCAA82500.020.1835421
TCGCACG1257.642546E-616.19861222
TATCAAC114200.014.5582862
ATCTCCG36450.014.38103910
ATCAACG114400.014.3361783
TCAACGC114950.014.3067274
CGGTTCG950.005376505714.21164745
CGCGATC950.005382010714.20945423
CAACGCA117300.014.0392845
AACGCAG121450.013.5966036
TGCTTGA21700.013.48032545
TCTATAC11150.012.5097523
ATAGGAC10550.012.3682263
CCGTCTT31800.012.31178644
CGTACTA42000.012.21386729
GACCGTA42550.012.10899426
ACCGTAC42800.012.03838527
CGAGACC43650.012.00940423
CCGATCG1506.459668E-411.99909518
Produced by FastQC (version 0.11.5)