FastQCFastQC Report
Thu 26 May 2016
SRR2094085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955844
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172930.21736223083308318No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC148580.18675579863054126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137970.1734196899788382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101730.12786826891025013No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC81040.1018622285705954RNA PCR Primer, Index 46 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109300.030.8901441
GTATCAA216100.022.3509351
TATCAAC298100.016.0223712
ATCAACG299850.015.8238193
TCAACGC301800.015.7513964
CAACGCA308450.015.4263935
AACGCAG314650.015.1438756
ATCTCCG48350.014.9829810
GGCGAAT49250.014.52855732
TATGCCG47350.014.3034243
GCGAATC50750.014.23215233
CTCGTAT48800.014.15488939
CGAATCT51250.013.96185134
TCTCGTA49700.013.94444838
AGGCGAA51750.013.91365231
TAAGGCG52100.013.86197929
TCGTATG50000.013.77017140
CGAGACT52500.013.75627723
TGCCGTC49800.013.64577845
AAGGCGA53300.013.63448330
Produced by FastQC (version 0.11.5)