FastQCFastQC Report
Thu 26 May 2016
SRR2094084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5664552
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC149640.2641691699537757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92880.1639670710057918No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC78780.13907542908953788TruSeq Adapter, Index 10 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71910.126947373772895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55700.030.8683361
GTATCAA115350.022.094741
TACGCCG600.006516111518.748745
TATACCG2800.018.48095
ATCTCCG44800.016.72320610
ATCAACG149900.016.5399133
TATCAAC152900.016.4213922
TCAACGC151300.016.3273874
CAACGCA154550.015.9840425
CTCGTAT46250.015.66464139
AACGCAG158150.015.6344216
TCTCGTA46350.015.53375738
TATGCCG46800.015.52958343
TGCCGTC48250.015.20266145
CGAGACC48800.015.07676123
ATGCCGT48850.015.06212744
TCGTATG48450.015.04649140
TCTCCGA50000.014.93899511
CGTATGC49300.014.83283741
ACGAGAC50400.014.82121722
Produced by FastQC (version 0.11.5)