FastQCFastQC Report
Thu 26 May 2016
SRR2094083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5260089
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93580.17790573505505325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75550.14362874848695525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.1071084538683661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53250.030.7707161
GTATCAA106800.021.567631
TATACCG2001.8189894E-1217.9983165
ATCAACG141900.015.981593
TATCAAC144400.015.8918652
TCAACGC142900.015.8225224
CAACGCA146850.015.4735275
AACGCAG149900.015.1586896
GTGGTAT21750.014.3002171
ACGCCCG2957.2759576E-1213.72844321
TCTCGTA17450.013.66764238
TGGTATC20350.013.4876812
TATGCCG17400.013.44880843
ATCTCCG17300.013.394710
TGCGTAG2703.2923708E-1013.33208714
CTCGTAT17700.013.09323939
ACGCAGA173550.013.0540947
TGCCGTC18300.013.03404545
TCGTATG18300.012.91022240
ATGCCGT18650.012.91009344
Produced by FastQC (version 0.11.5)