FastQCFastQC Report
Thu 26 May 2016
SRR2094082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7094603
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC129470.1824908314108626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84730.1194288108862469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.028.8381651
GTATCAA113950.021.480371
CGAGACT41100.016.91579423
TCTCGTA41300.016.67020638
ATCAACG143400.016.567963
CTCGTAT41850.016.45112439
TCAACGC144850.016.4487064
TATGCCG41600.016.44262943
TATCAAC146900.016.3570042
ACGAGAC43500.016.2410122
CCCACGA43750.016.14740819
TCGTATG43350.016.08982740
ATCTCGT43150.016.05988937
CAACGCA148400.016.0400645
ATCTCCG43500.016.03332710
CCACGAG43800.015.97510120
GCCCACG43950.015.97154418
CGTATGC43850.015.90647541
CACGAGA43700.015.85776221
TGCCGTC42550.015.7581345
Produced by FastQC (version 0.11.5)