FastQCFastQC Report
Thu 26 May 2016
SRR2094081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7203071
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147770.20514860953057384No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC134040.18608729526614412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115630.16052875225025548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84760.11767203183197833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83500.030.4996811
GTATCAA167650.021.6702921
ATCTCCG42300.016.2746610
TATCAAC221850.016.225252
ATCAACG221750.016.2021313
TCAACGC223350.016.0961384
AATACCG2101.07320375E-1016.0695175
CTCGTAT42600.015.84560639
TATGCCG42500.015.830605543
CAACGCA227950.015.8305375
TCTCGTA43150.015.54009738
AACGCAG232150.015.5344436
TGCCGTC44600.015.18675645
ATGCCGT44900.015.13498244
ATCTCGT45750.015.09991837
GCCCACG45600.015.04807418
CCGAGCC46850.015.03075214
TCGTATG45200.014.88445540
ACGAGAC47150.014.83937522
TCCGAGC46750.014.77364413
Produced by FastQC (version 0.11.5)