FastQCFastQC Report
Thu 26 May 2016
SRR2094080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5330830
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC141910.2662062005353763No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC94790.17781471178034192TruSeq Adapter, Index 11 (95% over 22bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA69500.1303736941526929TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56980.10688767040029414No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG54710.10262942168480331RNA PCR Primer, Index 11 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.026.204061
GTATCAA86550.020.80221
ATCAACG115000.015.3769213
TCAACGC116200.015.2374854
TATCAAC116650.015.197992
CAACGCA119150.014.8979885
ATCTCCG51350.014.67739710
AACGCAG122350.014.5267276
CGTGCGC4350.012.92990910
CGTACTA57200.012.82315229
GTATAAT16700.012.8184841
TCTCCGA58950.012.78514611
CCGTCTT40350.012.71443644
TCTCGTA58000.012.68578638
ACCGTAC57950.012.61836527
ACGCAGA140200.012.5488367
TATGCCG58700.012.45784443
GACCGTA58800.012.43595826
CGTCTTC41350.012.40845
CTCGTAT58600.012.40149439
Produced by FastQC (version 0.11.5)