FastQCFastQC Report
Thu 26 May 2016
SRR2094079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5959817
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75990.12750391496920124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60670.10179842770340096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52600.027.504861
GTATCAA93200.020.6007861
ATCAACG122150.015.5636823
TCAACGC123750.015.3260934
TATCAAC126450.015.1945592
ATCTCCG19000.015.03806410
CAACGCA126350.015.01071555
GGCGAAT17900.014.95832932
GCGAATC18050.014.83402233
AACGCAG130850.014.5632656
AATCTCG18650.014.47816336
TCTCGTA18800.014.12303138
TAAGGCG19450.013.99658629
CGAGACT19800.013.97619723
CGAATCT19350.013.95440634
CTCGTAT18750.013.92009639
TATGCCG18750.013.5608943
GCCCACG20850.013.4881718
TCCGAGC20850.013.48794313
AAGGCGA20650.013.40124130
Produced by FastQC (version 0.11.5)