FastQCFastQC Report
Thu 26 May 2016
SRR2094078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5546122
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82420.1486083429105959No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC72130.1300548383176569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65600.11828084560707464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46950.030.1443271
GTATCAA96050.022.5245131
ATCTCCG22650.017.48192610
TATGCCG22150.017.06613543
AATCTCG23100.016.9476536
CACGAGA23750.016.86306221
TCAACGC127100.016.72754
TCTCGTA23050.016.69157838
ATCAACG127350.016.6773
TATCAAC130700.016.4216822
CTCGTAT23450.016.40686239
CAACGCA130350.016.3276985
AACGCAG133000.016.002376
CCCACGA25000.015.92861819
TCGTATG23900.015.90980940
ACGAGAC24700.015.85011522
TCCGAGC25050.015.80701213
ATGCCGT23900.015.72280244
TGCCGTC23950.015.68997945
TCTCCGA25400.015.67777311
Produced by FastQC (version 0.11.5)