FastQCFastQC Report
Thu 26 May 2016
SRR2094077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6851889
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC123700.18053415634724962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80790.11790909047125545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60950.029.6551041
GTATCAA123550.021.5065041
CGCTATC34650.018.5052533
CGCTACG34550.018.49450328
TATCTCG35050.018.23027436
AACGTCG750.001111052917.99984422
CTCGTAT35300.017.8468739
TATGCCG35200.017.83495343
ACGCTAC36250.017.81441727
TGCCGTC35150.017.79682545
TCTCGTA35500.017.74632338
GACGCTA36700.017.65729526
CTACGCT36750.017.44857430
TACGCTA36800.017.36283731
ACGCTAT36900.017.3159132
ATGCCGT36000.017.18910644
GCTACGC37000.017.14824929
CGAGACG38450.017.08710123
GAGACGC38400.017.05137824
TCGTATG37500.016.91997540
Produced by FastQC (version 0.11.5)