FastQCFastQC Report
Thu 26 May 2016
SRR2094076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8273089
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC169240.20456687943282126RNA PCR Primer, Index 23 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96430.11655863970519355No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTC88110.10650193658015766RNA PCR Primer, Index 23 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78850.028.6952821
GTATCAA150450.021.5100141
ATCTCCG51600.016.7427610
ATCAACG198100.015.9792033
TATCAAC201000.015.8941692
TCAACGC200550.015.7839974
CAACGCA204400.015.4866955
CTCGTAT56500.015.21219839
AACGCAG210000.015.020156
TATGCCG56800.014.93469343
TCTCGTA57400.014.85608538
TGCCGTC56700.014.80301145
CGAGACC58650.014.69299723
TCCGAGC60100.014.56207613
CCCACGA60450.014.47767519
CACGAGA60050.014.38765421
CCACGAG60400.014.37817520
ACGAGAC60850.014.30942422
TCGTATG59950.014.26196340
TCTCCGA61050.014.188066511
Produced by FastQC (version 0.11.5)