FastQCFastQC Report
Thu 26 May 2016
SRR2094075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9693529
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC266730.27516294633254823RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177980.18360702278808885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143870.1484185996658183No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC142950.14746951290907576RNA PCR Primer, Index 39 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107360.11075429804769758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123400.030.9703461
GTATCAA242350.021.6644781
ATCTCCG80350.016.71586210
AACGTCG2057.4578566E-1116.46300522
TATCAAC323100.016.1335322
ATCAACG327550.015.8319233
TCAACGC328300.015.7614924
CAACGCA334550.015.4670385
TATGCCG87750.015.20655443
AACGCAG340900.015.185536
TCTCGTA87650.015.04264338
CGACGTT900.00375903114.99854412
CTCGTAT88350.014.92345939
TGCCGTC90000.014.72683745
TCGTATG90950.014.62113740
CACGAGA91800.014.55846221
CGAGACC92250.014.53629923
CCACGAG92250.014.53554920
CCCACGA93000.014.44222219
ATGCCGT91950.014.4390744
Produced by FastQC (version 0.11.5)