FastQCFastQC Report
Thu 26 May 2016
SRR2094074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8391210
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC221020.263394671328688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148630.1771258257152425No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC118700.14145754903047356TruSeq Adapter, Index 1 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117720.14028966025162046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102000.030.3362641
GTATCAA201850.021.7496341
TCAACGC263300.016.4482784
ATCAACG263800.016.4426883
TATCAAC266050.016.4135632
CAACGCA270050.016.070475
ATCTCCG65900.015.73814510
AACGCAG275400.015.7337756
TCTCGTA65200.015.49590638
TATGCCG65200.015.42559443
CTCGTAT65650.015.35413339
CGTATGC67400.015.15531641
TGCCGTC66400.015.11437445
TCGTATG67800.014.99998340
ATGCCGT67400.014.98912144
CGAGACT67650.014.96565323
ACGAGAC68550.014.768990522
CGAGCCC69950.014.73061815
CCACGAG68750.014.72602720
TCCGAGC71150.014.64010213
Produced by FastQC (version 0.11.5)