FastQCFastQC Report
Thu 26 May 2016
SRR2094073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5888527
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC100560.17077275862027974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86610.14708262354914903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68830.1168883151932563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.029.0903821
GTATCAA111650.020.9131321
ATCAACG142750.015.8549463
TCAACGC143200.015.8365444
TATCAAC143950.015.8321792
CAACGCA146400.015.4903935
AACGCAG149300.015.1895076
ATCTCCG32800.014.54135810
AATCTCG34300.013.97269136
TCTCGTA34100.013.79082338
CTCGTAT34350.013.68987239
CGCGGTT2002.4986548E-713.4987710
CGAGCCC35900.013.4739315
CCACGAG36600.013.4007620
CACGAGA36500.013.375833521
ACGAGAC36050.013.35557222
TCTCCGA36450.013.27050911
ACGCAGA171500.013.1970497
CCCACGA36800.013.14429519
ATGCCGT35800.013.13628544
Produced by FastQC (version 0.11.5)