FastQCFastQC Report
Thu 26 May 2016
SRR2094072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7308951
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC131820.18035419857103982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86170.1178965353578099No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC73720.1008626272087472RNA PCR Primer, Index 46 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.028.9665131
GTATCAA118100.021.7725891
ATCAACG153950.016.484143
TCAACGC155550.016.3290444
TATCAAC158550.016.0910242
CAACGCA161850.015.7212395
ATCTCCG44200.015.62618710
AACGCAG162850.015.5970726
GGCGAAT45400.014.86831532
GCGAATC45700.014.72147433
CTCGTAT44950.014.716516539
TCTCGTA45550.014.57266138
ACGAGAC48200.014.51640822
CGAATCT47150.014.45973134
AATCTCG47100.014.37953736
TGCCGTC44450.014.32700945
TATGCCG45400.014.32392743
CGAGACT47600.014.32136623
TAAGGCG47700.014.29183129
TCCGAGC48850.014.23084813
Produced by FastQC (version 0.11.5)