FastQCFastQC Report
Thu 26 May 2016
SRR2094071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7145288
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC437090.6117178201914325RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTC231670.32422765884314253RNA PCR Primer, Index 23 (95% over 24bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA155230.21724806613813186RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG138240.1934701582357492RNA PCR Primer, Index 23 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131840.18451320646557565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108390.15169437537017402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79330.11102421623872963No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT72120.10093365026014346RNA PCR Primer, Index 23 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104550.028.4045241
GTATCAA194150.020.3790191
ATCTCCG130650.017.70239610
TGCTTGA46550.016.1932645
TACACAT153600.015.9508925
TATACAC154050.015.9042993
ACATCTC152200.015.6837218
TATGCCG142600.015.57369143
CGAGACC146950.015.57181423
CTCGTAT143400.015.53322939
TCTCGTA143800.015.45872838
TGCCGTC142150.015.38609945
ATGCCGT143250.015.33088944
CGAGCCC151950.015.32455815
TCTCCGA151550.015.2907811
CCACGAG150000.015.28401120
ATCTCGT145300.015.2065537
CCGAGCC157600.015.20343714
GCCCACG152550.015.19065118
CACGAGA150700.015.18432621
Produced by FastQC (version 0.11.5)