FastQCFastQC Report
Thu 26 May 2016
SRR2094070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7260363
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC103200.1421416532479161RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96610.13306497209574783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80040.11024242176320936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70950.029.2565651
GTATCAA135650.020.984551
ATCAACG176950.015.7275073
TCAACGC177800.015.6396674
TATCAAC180450.015.5970042
CAACGCA180450.015.4349255
AACGCAG185250.015.0471366
TATACCG4400.014.828125
ATCTCCG33500.014.23743710
TCTCGTA35150.013.31418838
TATGCCG35150.013.2510943
ACGCAGA212200.013.0830977
CTCGTAT35850.013.05412839
CGAGACC36200.012.92818123
CGCAGAG215200.012.80662258
TCGTATG37600.012.68608740
CCACGAG37900.012.46624920
CGTATGC38150.012.44447441
TGCCGTC37600.012.328999545
ACGAGAC38600.012.29897222
Produced by FastQC (version 0.11.5)