FastQCFastQC Report
Thu 26 May 2016
SRR2094069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5736417
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99210.17294767796692606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82450.1437308340729065No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC71650.12490375089537598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58360.10173597909635929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56450.031.9439321
GTATCAA112150.021.8872381
TATCAAC152000.015.9260772
ATCAACG151650.015.814483
TCAACGC153450.015.6289734
GACCGTA1301.1089531E-515.5754027
CAACGCA156850.015.3332185
AACGCAG160750.015.00320156
CTCGTAT23800.014.55892639
ATCTCCG24800.014.15184410
GTGGTAT22300.014.049871
CGAGACT24650.013.96439723
TCGTATG25000.013.95009740
TGGTATC18900.013.9272122
TCTCGTA24900.013.73587438
TATGCCG25250.013.72334743
TATACCG4100.013.7181725
TATACTG18700.013.4746215
TGCCGTC26200.013.39844145
ATTATAC16300.013.3882643
Produced by FastQC (version 0.11.5)