FastQCFastQC Report
Thu 26 May 2016
SRR2094068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5434664
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC112800.20755652971370447RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95720.17612864383152296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77410.14243750855618673No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC60440.11121202709127925RNA PCR Primer, Index 27 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58460.10756874758034721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59750.031.1146891
GTATCAA117450.021.5080991
ATCTCCG36250.016.81879410
TATCAAC159950.015.7953442
TATCTCG37050.015.79030936
ATCAACG160000.015.6216773
TCAACGC160800.015.57193854
ACGGACT39200.015.38233527
CGGACTC39500.015.26522728
CAACGCA165200.015.1844275
GACGGAC39850.015.13143226
CACGAGA40500.015.05413721
CCACGAG41500.014.85456820
AACGCAG169000.014.8163786
CGAGACG41000.014.81581323
TATGCCG39950.014.81332143
ACGAGAC41550.014.78200122
TCTCGTA39300.014.6571338
GAGACGG41450.014.65590924
AGACGGA42150.014.57265325
Produced by FastQC (version 0.11.5)