FastQCFastQC Report
Thu 26 May 2016
SRR2094067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10468466
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1438151.3737924926154415No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1418651.3551651216138068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1162531.1105065441297701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910760.8700033032537909No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC705210.6736517079006609RNA PCR Primer, Index 26 (100% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA596050.5693766402833041No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA412180.3937348604848122RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG406940.38872935155924465RNA PCR Primer, Index 26 (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229530.21925848543616608No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211790.2023123540736532No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT207500.19821433245329353RNA PCR Primer, Index 26 (100% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164730.1573582987230412No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158930.15181784991229852No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148690.14203609201195286No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA132030.12612163042799204RNA PCR Primer, Index 26 (100% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123190.11767722224058424No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC104920.10022480848674487RNA PCR Primer, Index 26 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA529250.033.7605321
GTTCGAC11050.028.5044316
TCGACCG11100.028.37603418
CGACCGT11450.027.50864219
CTGCTTG73100.026.07141144
AAGTTCG12600.025.35504714
GCGTATC901.1723387E-825.0417081
TGCTTGA74900.024.7265945
GACCGTC13200.024.2026620
GATAGTC15100.022.6464847
CGCTCCG13900.022.501934
GTATCAA1057900.022.27551
TCCGCCA14100.022.02313837
GCGCTCC14700.021.276833
GCTCCGC15200.021.02151335
AGATAGT17400.020.2994636
CGTCACT11000.020.2837851
CGCTATC807.575016E-519.68596517
TAGCGGA807.5769625E-519.6853076
CAGCGCT16250.019.66263631
Produced by FastQC (version 0.11.5)