FastQCFastQC Report
Thu 26 May 2016
SRR2094066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6848511
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC142470.20803062154678584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134650.19661208107864614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104190.15213525976668507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77680.11342611554540834No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC75640.11044736585806754RNA PCR Primer, Index 17 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76550.031.4212231
GTATCAA160650.023.2371831
TATCAAC218550.016.8616682
ATCAACG220100.016.6509283
TCAACGC221750.016.5473234
CAACGCA226350.016.211045
ATCTCCG45150.015.74587310
AACGCAG233500.015.7242776
TATGCCG49350.013.95195643
AATCTCG51350.013.847445536
CTCGTAT50250.013.79142639
TCTCGTA50800.013.7756938
ACGCAGA266500.013.768747
ACGAGAC52850.013.53710822
CGCAGAG269250.013.5194878
CACGAGA52750.013.4348221
TCGTATG51700.013.40462540
CGAGACA54100.013.34908723
CCCACGA53300.013.33820219
TGCCGTC51550.013.31365845
Produced by FastQC (version 0.11.5)