FastQCFastQC Report
Thu 26 May 2016
SRR2094065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9102763
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC373090.4098645652973718No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC201130.22095489028990428RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155330.1706404967370896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126820.13932033603423488No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG124290.13654096014583705RNA PCR Primer, Index 46 (95% over 24bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA114950.12628033927720628RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110000.026.1056671
GTATCAA200000.019.925551
ATCTCCG121250.016.90346110
GGCGAAT127700.015.80486132
GCGAATC127850.015.78631933
AATCTCG128300.015.5910836
AGGCGAA129800.015.58382731
TCTCGTA126100.015.48854138
CGAATCT130350.015.48397534
TATGCCG125550.015.44902843
CTCGTAT126850.015.32542139
TCTCCGA134200.015.3226111
ATGCCGT127300.015.21880544
CGTATGC128200.015.16412241
ACGAGAC134600.015.12697522
TGCCGTC128050.015.09527145
CGAGACT135000.015.06557223
CGAGCCC136250.015.04269515
CCCACGA135300.015.01529119
ATCAACG260350.015.001383
Produced by FastQC (version 0.11.5)