FastQCFastQC Report
Thu 26 May 2016
SRR2094064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6150170
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79780.1297199914799103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63960.10399712528271576No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC63090.10258253023900153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49450.029.984461
GTATCAA97900.021.475121
TAGCGCG550.003940289420.4529024
ATCAACG130700.015.7160283
TCAACGC131950.015.4989424
TATCAAC134300.015.4120152
CAACGCA134300.015.2277415
AACGCAG137450.014.8460246
CTCGTAT22150.014.52573639
ATCTCCG22850.014.37521210
TCTCGTA23050.014.15379538
GCCGTCT16400.014.1315743
TCTCCGA23900.014.02606911
CGAGACG24100.013.63082923
CCGTCTT17300.013.52668344
GACGTAG23850.013.39678326
AGACGTA24800.013.24651925
ATGCCGT24000.013.21940744
AATCTCG24950.013.16591636
CGAGCCC24950.013.16527415
Produced by FastQC (version 0.11.5)