FastQCFastQC Report
Thu 26 May 2016
SRR2094063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6589595
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC122410.1857625544513737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106060.16095071093140018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85780.13017491970295594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60250.030.1095471
GTATCAA122400.022.0567131
ATCAACG159150.016.5536373
TCAACGC160000.016.4516324
TATCAAC162850.016.32952
CAACGCA163550.016.108295
AACGCAG167550.015.75058656
ATCTCCG38050.015.60962210
TCTCGTA40450.014.29510538
AATCTCG40750.014.24475436
TATGCCG40050.014.21424243
CTCGTAT40400.014.09003239
ATACCGA1605.0395756E-614.0612246
TCTCCGA42700.013.90974511
TGCCGTC41600.013.90098445
ATGCCGT41250.013.855391544
ATCTCGT42100.013.78839337
ACGCAGA190400.013.7303727
ACGAGAC43100.013.72970422
TCGTATG41800.013.61832140
Produced by FastQC (version 0.11.5)