FastQCFastQC Report
Thu 26 May 2016
SRR2094062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006402
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC29290.14598270934737906RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTC27470.13691174550264604RNA PCR Primer, Index 23 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.11792252998152912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.11523114510452043No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA20530.10232246578701576RNA PCR Primer, Index 23 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.026.9071181
TGAACCG709.318064E-725.7127915
GTATCAA31050.022.1953011
CGGTAGG600.00651339918.74937631
GTCGCGT1053.115848E-517.1418616
CGCGTCC1053.115848E-517.1418618
TCGCGTC952.8219205E-416.57798417
GGTCGCG1104.6425965E-516.36268615
ATACCGT1104.6425965E-516.3626866
ATCAACG48350.016.1934742
TATCAAC48450.016.1765791
TCAACGC49200.015.9137083
CAACGCA50550.015.5332214
AACGCAG52650.015.1273255
TGGACCG900.003756672614.9991285
TACACCG1056.0423074E-414.9991275
AACCGCG950.00537993614.2097017
CTGCGCA1504.2759508E-513.4992159
TCCGTGA1000.00755650113.49921510
CTAGTAC3051.2732926E-1113.2779163
Produced by FastQC (version 0.11.5)