FastQCFastQC Report
Thu 26 May 2016
SRR2094061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10215836
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC217760.21315925588468726RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC120350.11780729447888552RNA PCR Primer, Index 39 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96200.029.4469071
GTATCAA181000.021.042031
ATCAACG243950.015.3277313
TATCAAC246300.015.2819652
TCAACGC245050.015.2405634
CAACGCA249950.014.9237885
AACGCAG255650.014.6174466
ATCTCCG72050.014.08283810
GTACATG351500.013.9768931
TACATGG347350.013.8350782
CGTGCGC8250.013.36256410
TAAGGCG8450.013.046295
ACATGGG358900.012.92596153
CTCGTAT76350.012.84844739
TATGCCG79350.012.59038443
TCTCGTA78000.012.57665338
CGAGACC80750.012.53879623
TCGTATG79700.012.42149540
ATGCCGT80100.012.36055544
ACGCAGA301750.012.3395227
Produced by FastQC (version 0.11.5)