FastQCFastQC Report
Thu 26 May 2016
SRR2094060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10022325
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC521210.520048990628422RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC268800.2682012407300701RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257880.25730556532541105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206380.2059202829682733No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA172770.172385150152285RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG158840.158486179604034RNA PCR Primer, Index 27 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145410.14508609529226002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172250.028.6138531
GTATCAA334200.021.3294431
ATCTCCG149650.017.46910
TATCAAC444750.015.898982
ATCAACG448900.015.6968673
TCAACGC451300.015.5984364
TATCTCG162050.015.56547636
TATGCCG162200.015.53720943
TCTCCGA167000.015.5193911
CGAGACG166600.015.43557823
ACGGACT163850.015.42032927
TCTCGTA163900.015.38978238
CAACGCA460700.015.2899375
CTCGTAT164900.015.282239
CGGACTC165150.015.27147128
TGCCGTC163550.015.217257545
AACGCAG463500.015.2024246
CGTATGC166250.015.17178841
ATGCCGT163750.015.170279544
GACGGAC167800.015.08407626
Produced by FastQC (version 0.11.5)