FastQCFastQC Report
Thu 26 May 2016
SRR2094059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225749
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC326610.39705806729575627No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC185560.22558432064970615RNA PCR Primer, Index 17 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166340.20221866726057408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131010.15926817120240358No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA127370.15484304225669904RNA PCR Primer, Index 17 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG111500.13554996633133348RNA PCR Primer, Index 17 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92530.11248823663352722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108750.030.3810541
GTATCAA215350.022.0818611
ATCTCCG97700.016.57943710
TATCAAC296800.015.9939512
ATCAACG295900.015.9817713
TCAACGC297500.015.9033824
CAACGCA304750.015.5324225
AACGCAG312700.015.1591146
CTCGTAT108650.014.55819839
TATGCCG109100.014.51992143
TCGTATG109650.014.48698940
TCTCGTA109950.014.44842638
TCTCCGA112600.014.42558111
TGCCGTC109800.014.34651545
CACGAGA112000.014.32251421
CGTATGC111100.014.27792341
GCCCACG113800.014.2736418
TCCGAGC114350.014.22448813
ACCGTAT3800.014.2090438
AATCTCG113100.014.20500636
Produced by FastQC (version 0.11.5)