FastQCFastQC Report
Thu 26 May 2016
SRR2094058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593374
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC100870.18033837894623173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.028.3164351
GTATCAA91350.021.2432251
ATCAACG121600.015.4484913
TATCAAC123100.015.424732
TCAACGC122150.015.3789324
ATCTCCG31750.015.37620610
CAACGCA124550.015.1187165
GTACGGG1806.744449E-815.0256961
GACCGTA32500.014.67630626
AACGCAG129950.014.5077786
CGTACTA32750.014.42648729
CTCGTAT33400.014.41671939
ACCGTAC33000.014.31757927
TATACCG2702.1827873E-1114.1650565
TCTCGTA34800.013.96630238
CCGTACT34300.013.84015428
TATGCCG35300.013.83222243
TCGTATG35400.013.60221640
GATCTCG35750.013.59541336
TGCCGTC35700.013.4892445
Produced by FastQC (version 0.11.5)