FastQCFastQC Report
Thu 26 May 2016
SRR2094057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5959617
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC117860.19776438653692005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109010.18291443896478582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88980.1493048966066108No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC69290.11626586070883414RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63930.10727199415667148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.030.5037751
GTATCAA133150.020.6000791
CGCGATC1703.6379788E-1219.8516223
CCGCGAT1802.0918378E-1017.49868822
TATCAAC183750.014.8393312
ATCAACG182850.014.7647243
TCAACGC184600.014.6125684
TCGCATA1108.6001126E-414.31722823
CAACGCA189150.014.2610635
CGAACGT2702.1827873E-1114.16881645
ATACCGA2408.076313E-1014.0610916
ATCTCCG44250.014.03249510
AACGCAG193150.013.9773736
GTGGTAT27050.013.6645731
CGATCGT2501.4879333E-913.49921425
GGCGAAT45150.013.30643732
TGGTATC25500.013.2339682
GCGAATC46100.013.08103733
CTCGTAT44700.012.98647539
TCTCGTA46300.012.87851738
Produced by FastQC (version 0.11.5)